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ctv rosa26 locus targeting vector  (Addgene inc)


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    Structured Review

    Addgene inc ctv rosa26 locus targeting vector
    Ctv Rosa26 Locus Targeting Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ctv+rosa26+locus+targeting+vector/CTV+(Plasmid+%2315912)/pmc06195819__NIHMS969746___supplement___1-103-34-39
    Average 93 stars, based on 41 article reviews
    ctv rosa26 locus targeting vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Transgenic Assay:

    Article Title: miR-30 Disrupts Senescence and Promotes Cancer by Targeting both p16 INK4A and DNA Damage Pathways
    Article Snippet: As a positive control for apoptosis, cells were treated with 3 μM Thapsigargin (Sigma) for 12 h. Each experiment was performed in duplicates. .. 13 Generation of miR-30c-1 transgenic mice To generate miR-30c-1 transgenic mice, we cloned 4 tandem copies of a miR-30c-1-coding genomic DNA fragment (miR-30c-1 hairpin plus 150 bp flanking sequences on each side) into the CTV Rosa26 locus targeting vector (Addgene plasmid #15912) (20), downstream of a loxPflanked STOP cassette containing 4 tandem copies of SV40 polyadynelation sites. .. The miR-30c1 sequence was amplified by PCR from pLV-miR-30c-1-puro (Biosettia) using primers LV-toCTV-f (5’-AGTTATGGGCGCGCCGCGCCGTCCAGGCACCTCGATTAGTTC-3’) and LVto-CTV-r (5’-CGGCCGCGGCGCGCCCCCAACCTAAAGACGACGTACTCCAAAAGC-3’) and cloned into the AscI site in CTV.

    Clone Assay:

    Article Title: miR-30 Disrupts Senescence and Promotes Cancer by Targeting both p16 INK4A and DNA Damage Pathways
    Article Snippet: As a positive control for apoptosis, cells were treated with 3 μM Thapsigargin (Sigma) for 12 h. Each experiment was performed in duplicates. .. 13 Generation of miR-30c-1 transgenic mice To generate miR-30c-1 transgenic mice, we cloned 4 tandem copies of a miR-30c-1-coding genomic DNA fragment (miR-30c-1 hairpin plus 150 bp flanking sequences on each side) into the CTV Rosa26 locus targeting vector (Addgene plasmid #15912) (20), downstream of a loxPflanked STOP cassette containing 4 tandem copies of SV40 polyadynelation sites. .. The miR-30c1 sequence was amplified by PCR from pLV-miR-30c-1-puro (Biosettia) using primers LV-toCTV-f (5’-AGTTATGGGCGCGCCGCGCCGTCCAGGCACCTCGATTAGTTC-3’) and LVto-CTV-r (5’-CGGCCGCGGCGCGCCCCCAACCTAAAGACGACGTACTCCAAAAGC-3’) and cloned into the AscI site in CTV.

    Plasmid Preparation:

    Article Title: miR-30 Disrupts Senescence and Promotes Cancer by Targeting both p16 INK4A and DNA Damage Pathways
    Article Snippet: As a positive control for apoptosis, cells were treated with 3 μM Thapsigargin (Sigma) for 12 h. Each experiment was performed in duplicates. .. 13 Generation of miR-30c-1 transgenic mice To generate miR-30c-1 transgenic mice, we cloned 4 tandem copies of a miR-30c-1-coding genomic DNA fragment (miR-30c-1 hairpin plus 150 bp flanking sequences on each side) into the CTV Rosa26 locus targeting vector (Addgene plasmid #15912) (20), downstream of a loxPflanked STOP cassette containing 4 tandem copies of SV40 polyadynelation sites. .. The miR-30c1 sequence was amplified by PCR from pLV-miR-30c-1-puro (Biosettia) using primers LV-toCTV-f (5’-AGTTATGGGCGCGCCGCGCCGTCCAGGCACCTCGATTAGTTC-3’) and LVto-CTV-r (5’-CGGCCGCGGCGCGCCCCCAACCTAAAGACGACGTACTCCAAAAGC-3’) and cloned into the AscI site in CTV.



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